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Pull down assay for GPX4 PC12 cells were treated with DAQ probe (DA-yne probe contains FeCl 3 solution) or DMSO for 6 hours, and then were harvested and lysed with RIPA buffer supplemented with protease inhibitor cocktails and phosphatase inhibitor cocktails

385 Mito-TEMPO (MT), another mitochondrial superoxide scavenger, also prevents muscle atrophy via alleviating mitochondrial dysfunction, oxidative stress and inflammatory

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Because glutathione is used up as it does its job, levels are sustained over time rather than fixed in a single visit

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