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The mNSC were cultured in Dulbeccos Eagles Medium (DMEM) (Lonza Walkersville, MD) supplemented with 10% fetal bovine serum, 5% horse serum, and 1% each penicillin-streptomycin (GIBCO, Invitrogen, Carlsbad, CA) and sodium pyruvate

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"I emphasize 'can' it's not even clear to the best scientists what are the triggers that allow that progression to happen," he said, noting that why some people have a higher risk than others of developing liver disease from drinking is not understood medically or biochemically

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