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Mol Cell Biochem 266:3756 Valko M, Rhodes CJ, Moncola J, Izakovic M, Mazura M (2006) Free radicals, metals and antioxidants in oxidative stress-induced cancer

To investigate the requirement of ROS for cell death, we used ROS scavengers

These effects were associated with the inhibition of NF-B nuclear translocation and reduced phosphorylation of B and mitogen-activated protein kinases, including p38, extracellular signal-regulated kinases 1 and 2 (Erk 1/2), and c-Jun N-terminal kinase (282)

However, there may be upregulation of succinate and aspartate (Zhou et al., 2018)

Statistical analysis The statistical analysis of the results was performed on Microsoft Excel 2016 (Microsoft Inc., Office 2016)

Cells were then incubated with or without cisplatin, etoposide (Cell Signaling Technologies, 2200S), doxorubicin (Sigma-Aldrich, D1515), staurosporin (Cell Signaling Technologies, 9953) or paclitaxel (Cell Signaling Technologies, 9807S) for an indicated period