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Poddar, Theophylline-induced Changes in Mammalian Adenosine Deaminase Activity and Corticosterone Status: Possible Relation to Immune Response, Methods and Findings in Experimental and Clinical Pharmacology, April 1997;19(3):18184

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Add labelled substance and measure labelled products Approaches in present pyrolysis study Develop pyrolysis to simulate conditions during smoking Use pyrolysis to measure amount of decomposition during smoking SOME DEFINITIONS (1) Pyrolysis: Decomposition due to heat Pyrosynthesis: Thermal decomposition of substance followed by reaction of their decomposition products to form new, larger molecules SOME DEFINITIONS (2) Pyrolysis in inert atmosphere: Thermal decomposition, pyrosynthetic reactions can occur Pyrolysis in atmosphere containing oxygen: Combustion reactions can also occur Sometimes called oxygen-sensitised or combustion-sensitised pyrolysis Smoke Volatile gases Gases Pyrolysis Distillation Char Oxidation Tobacco Ash Residual Char Loss Feedback Heat Distillation-Pyrolysis Zone Combustion Zone Air PYROLYSIS Pyrolysis techniques used in many studies over many years to establish component-smoke product relationships Many false relationships published Laboratory pyrolysis conditions must match combustion conditions inside cigarette Example (1) of a False Pyrolysis Relationship Schmeltz & Schlotzhauer (1968) pyrolysed menthol at 600C & 860C They found 22% & 84% pyrolysed respectively The pyrolysis products included phenol & benzo[a]pyrene BUT smoking of cigarettes containing radiolabelled menthol, shows that 99% of the menthol transfers to the mainstream intact