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Metabolically activated tobacco smoke carcinogens directly cause mutations observed in tumor suppressor genes and oncogenes (Vaidya et al., 2020)

Luminex analysis A custom magnetic Luminex assay panel from Life Technologies was used as previously described 20 to assess the levels of the following 15 cytokines and chemokines in human plasma: IL-1, G-CSF, IL-10, IL-13, IL-6, IL-17, MIP1, VEGF, IFN, IL-12p70, IFN, IL-1RA, TNF, IL-4 and IL-8

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[DOI] [PMC free article] [PubMed] [Google Scholar] [252].Caliri AW, Caceres A, Tommasi S, Besaratinia A, Hypomethylation of LINE-1 repeat elements and global loss of DNA hydroxymethylation in vapers and smokers, Epigenetics, 15 (2020) 816829

[i] "Two cigarettes a day are enough to make a teenager permanently dependent" [i] DNF - For a Zero Tobacco World |

Human subject approval will be obtained before data collection, and formal informed consent obtained from participants