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PMID: 30587505

Vitamin C is a strong antioxidant

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To assess cellular DNA content, cells were incubated for 72 h with BCT1028 (40 M), PMA (1 10 6 M), or v/v DMSO (control), fixed in 70% ethanol overnight at 4 C, washed with D-PBS (Dulbeccos phosphate-buffered saline), and incubated in 500 l Propidium Iodide (PI)/RNAse Staining Buffer (BD Pharmingen