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Lysates were combined with 6 Laemmli SDS sample buffer (Boston BioProducts, BP-111R) containing 5% 2-mercaptoethanol (VWR Life Science, M131), then loaded onto 420% Criterion TGX pre-cast gels (Bio-Rad, 5671093)

Here are some specific groups who may benefit: Athletes often experience injuries and require faster recovery times

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The signal peptide (red-orange) at the N-terminal region indicates the sequence responsible for targeting the protein to specific cellular locations