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Euthanasia At the end of the experiment, rats were euthanized by an overdose of sodium pentobarbital (150 mg/kg, intraperitoneally), consistent with the AVMA Guidelines for the Euthanasia of Animals

intragastrically administered 320 mg/kg WECP in 200 L saline)

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The injection involved inserting the needle from the lower pole to the upper pole of the kidney and gradually withdrawing it after administering the rAAV9 solution

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