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Many of the Aka men think of cannabis as a performance-enhancing drug, says anthropologist Edward Hagen, who led the study

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Funding This masters research on which this article is based was partly funded by The Global Tobacco Control Research Programme (GTCRP) of Simon Fraser University led by Professor Kelley Lee

Immunostaining of HBE cells ALI cultures were fixed in 4% paraformaldehyde 20 min at RT, followed by incubation in permeabilization solution (0.3% Triton X-100/PBS) for 15 min and in blocking solution (5% FBS/PBS/3% BSA) for 1 h at RT, incubated in primary antibodies overnight at 4 C, washed, incubated in secondary antibodies for 2 h at RT, washed, and then mounted for imaging