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The PVDF membrane was then subjected to blocking with 5% milk and subsequently incubated with the designated primary antibodies overnight at a temperature of 4 C: -actin (Proteintech, 66009-1-lg) (1:50,000), SIRT1 (Abcam, ab189494) (1:1000), NF-B p65(CST, 8242) (1:10,000), NF-B p65 (acetyl Lys310) (Abcam, ab19870) (1:10,000), Type II Collagen Alpha 1 Chain (COL2A1) (Proteintech, 28459-1-AP) (1:1000), Ferritin heavy chain 1 (FTH1) (Abcam, ab183781) (1:1000), Glutathione peroxidase 4 (GPX4) (Abcam, ab125066) (1:1000), acyl-CoA synthetase long-chain family member 4 (ACSL4) (Proteintech, 22401-1-AP) (1:5000), P300 (Santa Cruz, sc-48343)(1:1000)

These results demonstrate that multi-analyte sensing can improve the early diagnostic sensitivity of kidney disease by capturing the interconnected metabolic changes in the body

These problems are caused by various phytopathogenic organisms, such as microorganisms and insects, each with different infection, development, and survival strategies within the host (Meng et al., 2009

Adeyemi OS, Elebiyo TC

(2024) using a Mini-PROTEAN Bio-Rad system

Additionally, we discovered that a GSH threshold of 10.2 nmol/mg effectively differentiates resistant cells from sensitive cells, with an AUC of 0.913, providing a robust distinction between chemotherapy-resistant and sensitive patients