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(8)Fmoc-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- (8) Preparation of Fmoc-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin: (7)Fmoc-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-1050530105010603DMF3DMF2DMFFmoc-Pro-OHDIEATBTU/HOBtHBTU/HOBtBOP/HOBt105010603DMF3DMF2Fmoc-Pro-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val- In the Fmoc-Ala-Asp(otBu)-Asp(otBu)-Ala-Gly-Leu-Val-resin of step (7), add decapping reagent 1050 and react for 530 minutes, dry with vacuum pump, re- Add capping reagent and react at 10-50C for 10-60 minutes, pump dry, wash with isopropanol for 3 times, DMF for 3 times, distilled DMF for 2 times, add Fmoc-Pro-OH and DIEA dissolved in DMF , a mixture of TBTU/HOBt or HBTU/HOBt or BOP/HOBt, react at 10-50C for 10-60 minutes, dry it with a vacuum pump, wash 3 times with isopropanol, wash 3 times with DMF, wash 2 times with re-distilled DMF time, drained to obtain Fmoc-Pro-Ala-Asp (otBu)-Asp (otBu)-Ala-Gly-Leu-Val-resin

TB500: Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Lys (or full TB4)