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For the NF-B assay, an aliquot of the nuclear extract containing 8 g of protein was incubated with 32 P-end-labeled 22-mer double-stranded NF-B oligonucleotide (Promega Corporation, Madison, WI) with the NF- B consensus sequence: 5-AGTTGAGGGGACTTTCCCAGGC-3 and 3-TCAACTCCCCTGAAAGGGTCCG-5 for 30 min at 37C

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She, too, started out as a country artist who gradually crossed over into pop, and for a while, really made it work

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This thought discomfited me for a while, but as the weeks passed I began to indulge in something that academics who study vacations call rosy retrospection. The photographs came back, and we all looked very jolly in them