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In general, the amino heterocycle strongly contributes to binding, in fact, the positively charged amino group forms a salt bridge with Glu467 (N-O distance: 2.7 for piperazine, 3.6 for piperidine), while the other main interactions are hydrophobic and involve Ile339, Phe396, Leu399 (Figure 4B)

Proc Natl Acad Sci USA 110:31853190

Using our approach, a refolding trial can be completed in a single day compared to typically more than one week required for the dilution (if the IB washing steps are counted) or the dialysis methods for many receptor proteins (Fig

BmK AGAP, a potential candidate molecule, has demonstrated heterologous expression and maintained its pharmacological effects while increasing its yield

doi: 10.1016/j.freeradbiomed.2018.03.005 3 KuksalNChalkerJMaillouxRJ

Some CBS variants cause the enzyme to work too quickly, rapidly depleting homocysteine by shunting it down the transsulfuration pathway