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The following linear gradient program was used for the separation, with a total run time of 15 min

Finally, we would also like to inform all citizens that as of the current date, March 1st, 2012, the Locust Police Department is NOT conducting any fund raising activities

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The cells were stained for 2 h at RT with anti-human E-cadherin antibody (Invitrogen, CA, USA) that was diluted in PBST containing 1% BSA

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