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Mulder DW, Kurland LT, Offord KP et al (1986) Familial adult motor neuron disease: amyotrophic lateral sclerosis

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NAC containing fractions were pooled and dialyzed (M w cutoff 1214 kDa) over night against buffer B (20 mM HEPES pH 8.0, 50 mM NaCl, 2 mM CaCl 2 ) in the presence of thrombin (Merck) to remove the His 6 -tag

& Hornef, M

The total time of immobility was measured during the 6-min period of the testing session