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0.2 ml) were then added to each suspended pellet prior to sonication (4 1 min, followed by 2 min of incubation in ice) by means of a MiniBead-beater (Biospec Products, Bartlesville, OK, USA)

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1 (they need not all be determined by SEP), then adjusting for them in stratified analyses will remove part of the effect of interest, while stratifying without adjustment will induce collider bias, and estimates will be biased either way [38, 39]

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