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In ALI conditions, however, the puffing profile, number of puffs, dimensions of the culture wells, length of tubing carrying the smoke/vapor, temperature, and humidity are some of the factors that impact the physicochemical properties of the aerosol and ultimately the biological responses induced

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In these individuals, the nervous system may already be struggling with excitatory overload, poor GABA balance, inflammation, impaired detoxification, or limbic system hypervigilance

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