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[3] Keep the pack pressed onto the burn for 10 minutes at a time, or until it starts to feel uncomfortable

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This is hard to do in a standard human clinical study where one compares patients with a history of smoking versus those who do not smoke because all of the patients have different histories, backgrounds, and patterns of exposure to the stimulus. The study was also authored by Mariko Hirano-Kobayashi, a past research fellow with Ingber at the Wyss Institute and Boston Childrens Hospital, Wyss research assistants Youngjae Choe and Thomas Ferrante, present and former Wyss senior staff scientists Rachelle Prantil-Braun and Anthony Bahinski, and James C