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The advantage of our test is, therefore, that it shows changes in GR activity not only due to higher/lower GR gene activity but also due to the absence of the reaction cofactor NADP

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The proton leak across the mitochondrial inner membrane

Lowering the temperature will slow down the binding kinetics, so the incubation time should also be increased

Other factors which involve a lack of information and potentially contribute to heterogeneity include concomitant illnesses and medications, both of which may affect antioxidant status

ACG Clinical Guidelines: Prevention, Diagnosis, and Treatment of Clostridioides difficile Infections [published correction appears in Am J Gastroenterol