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2.9 TEM Pioloform-coated nickel grids (Plano, Wetzlar, Germany) were incubated with the anti-TMV antibody mAb75 ( 2.10 Cryo-TEM Purified VLPs were plunge-freezed in 180 C cold ethan on lacey carbon nickel grids (Science Services, Munich, Germany) and analysed with a cryo-TEM Hitachi HT7800 transmission electron microscope (Hitachi, Tokyo, Japan) operating at an acceleration voltage of 120 kV

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