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PCR reactions were initialed at 95C for 10 min, followed by 55 cycles of denaturing at 95C for 10 s, annealing at 60C for 30 s, and finally a cooling step at 4C

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Figure 4 shows the integrated network of cell death pathways triggered by Erastin in endometriosis, illustrating how ferroptosis, necroptosis, and pyroptosis are interconnected and modulated by autophagy-dependent processes