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Cells were cultured on a gelatin (0.02% (wt/vol))/fibronectin (10 g ml 1 ) matrix and were maintained in Claycomb medium (JRH Biosciences) supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, 0.1 mM norepinephrine, 100 U ml 1 penicillin and 100 g ml 1 streptomycin

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