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Lonza, Rockland, ME, USA) [30] or an epifluorescence microscope (Olympus BX53 or Zeiss Axioscope) after staining of a 1 ml subsample with DAPI (4,6-diamidino-2-phenylindole, final concentration 0.01 g/ml [31])

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Taking more vitamin D than the recommended amount, especially through supplements, can lead to side effects, including digestive discomfort like gas and bloating

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Protocol as for acetaminophen toxicity: 140 mg/kg IV initially: slow bolus not constant rate infusion (ammonia toxicity) then 70 mg/kg IV or PO x 3-6 (or more) treatments given at 8 to 12 hour intervals

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