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In total, 1 L of cDNA, together with 5 L of SYBR green mix (TIANGEN Biotech, Hainan, China), 0.3 L each forward and reverse primer, were added into the PCR mixtures (with the total volume of 10 L)

PMC 3967529

N-acetylcysteine N-acetylcysteine (NAC) is a modified form of L-cysteine and it is a widely used therapeutic agent, especially for mucolytic therapy and treating paracetamol overdose