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Thirty days after the six week smoking period of the trial, around 80 percent of the participants took part in a follow-up phone call

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qPCR was performed using TaqMan Gene Expression Assays (Thermo Fisher Scientific, Waltham, MA, USA) for FOXJ1 (Hs00230964_m1), IL6 (Hs00985639_m1), IL8 (Hs00174103_m1), KRT5 (Hs00361185_m1), MMP9 (Hs00234579_m1), MUC5AC (Hs01365601_m1), SCGB1A1 (Hs00171092_m1), and TGFB1 (Hs00998133_m1)

About 21 million American citizens suffer from at least one type of addiction, and the number of deaths has tripled over the past 30 years (Yerby, 2021)

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