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For specific staining of the dead cells after expression of WD-associated G53S or M128V PrP variand, control, and treated cells were washed in warm media and incubated for 1 h at 37 C in the incubator with BOBO3 (Life Technologies) diluted 1:1000 in the media

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10.1016/j.cell.2022.09.008 235 ZhaoB.LiL.WangL.WangC

3 It also promotes proliferation and reduces apoptosis of pancreatic -cells, thereby maintaining their quality and functionality

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