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Each tissue block was sliced (diameter: 0.6 mm), followed either by fixation with 4% paraformaldehyde (for IHC staining) or by freezing with 0.1 M phosphate buffer (for ELISA)

10.7554/eLife.10727 Elife

B., Lesley R., Schubert D

Fibromyalgia is the third most frequent musculoskeletal condition, and its prevalence increases with age

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To determine the optimal quantities of the three cell types required for periportal assembloid formation, we first investigated the proportions of portal fibroblasts and ductal cells in healthy human periportal liver tissue