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Some regular smokers believe smoking eases anxiety and they report this is a reason they continue to smoke

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Following saponin treatment, muscle fibers were rinsed twice in ice-cold mitochondrial respiration fluid (MIR05, in mM: 110 Sucrose, 0.5 EGTA, 3 MgCl 2 , 60 K-lactobionate, 20 taurine, 10KH 2 PO 4 , 20 HEPES, BSA 1 g.L 1 , pH 7.1) for 10 min each