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Nicotine, Quinoline (Internal standard), flavors (Eucalyptol, Camphor, Menthol, Methyl Salicylate, Ethyl salicylate, Cinnamaldehyde, Eugenol, Diphenyl ether, and Coumarin) and its internal standard (3, 4-(Methylenedioxy) acetophenoneMDA) were procured from Sigma Aldrich, USA having purity of 99.0%)

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