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For all interventions, the same questionnaires and measurements had to be completed at eight different assessment points: immediately prior to the interventions, during the interventions after 2, 4, 6 and 8 min, end of the interventions (after 10 min), and 10- and 20-min post-treatment

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Thus, these individuals presumably would not have had any change in potential CO protection or ANA prevalence between Periods 1 and 2, since vaping did not begin until 2007, but would have had a decrease in potential CO protection and, consistent with our hypothesis, a corresponding increase in ANA prevalence between Periods 2 and 3, as some of them took up vaping

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Over time, as your body adjusts to vaping, you might find that you need fewer puffs to satisfy your cravings compared to when you first made the switch from smoking