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Then, 30 ml of lentivirus supernatant was concentrated by centrifugation at 20,000 g at 4 C for 2 h and then resuspended in 600 l of StemMACS HSC expansion medium (Miltenyi Biotec)

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doi: 10.1136/jnnp-2019-320912 144 RaisonCLRutherfordREWoolwineBJShuoCSchettlerPDrakeDFet al

Western blot analysis was performed to detect the associated proteins

Then the slides were washed and prepared for the second round of fluorophore hybridization and imaging, these last steps were repeated for a total of four rounds