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However, later studies with ToMV using both fluorescence microscopy and biochemical fractionation methods determined that the replication proteins and replicase activity were associated predominantly with the vacuolar membrane, although they also showed some localization and activity with other less defined membrane fractions which included the ER (Hagiwara et al., 2003)

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Again, note that the smoking-cessation program alternative is made available without the participant having to demonstrate that it would be unreasonably difficult due to a medical condition, or medically inadvisable, to stop tobacco use (see Example 6

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