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Ptc, which is only expressed in a narrow stripe of cells just anterior to the AP compartment boundary by sensing the highest level of Hh, is a direct readout of Hh signalling

doi: 10.1007/s11302-021-09822-6

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Assay equivalency was verified by participation in the SARS-CoV-2 Neutralizing Assay Concordance Survey (SNACS) run by the Virology Quality Assurance Program and External Quality Assurance Program Oversite Laboratory (EQAPOL) at the Duke Human Vaccine Institute, sponsored through programs supported by the National Institute of Allergy and Infectious Diseases, Division of AIDS

Median symptom onset-to-groin puncture was 168 minutes

While heat causes gradual denaturation, freezing causes immediate mechanical damage that is far worse