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pH For pH quantitation, deionized water was added to tobacco, after shaking it and letting it rest in the dark for 45 min, we decanted the supernatant and measured pH using a pH electrode with and internal reference of Ag/AgCl, previously calibrated with buffers of 4, 7, and 10 pH levels

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Although smoke has been shown to cause apoptosis of HBE cells 5,6,7 , apoptosis is classically considered a non-inflammatory form of programmed cell death 8