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RNA isolation and qPCR analysis Total RNA from skin and lungs was isolated by RNeasy Fibrous tissue mini kits (Qiagen, Germantown, MD, 74,704)

Competitive endogenous RNAs (ceRNAs) act as sponges for miRNAs, ceRNAs can bind to miRNAs and reduce the abundance of miRNAs in the cell, thus reducing the inhibitory effect of miRNAs on target genes

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Call 911 or seek immediate medical attention if injection site bruising is accompanied by widespread hives, difficulty breathing, facial swelling, dizziness, or rapid heart ratethese may indicate anaphylaxis requiring emergency treatment

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If a vitamin deficiency persists over a long period of time and remains undetected, nerve damage can occur, triggering neuropathies (nerve pain) [3]