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Iron is tightly controlled at the systemic and cellular levels to prevent iron overload and toxicity, which is associated with liver disease, diabetes, osteoporosis, heart disease, atherosclerosis, and neurodegenerative diseases (Galaris et al., 2019

This is essential for proper reconstitution

Caspase-Glo reagent was added to the wells according to the manufacturers instructions and luminescent signal was measured on a SpectraMax M3 microplate reader (Molecular Devices)

Chronic obstructive pulmonary disease (COPD), smoking, and mechanical ventilation weaken the epithelial integrity of the respiratory tract