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It inhibits NNMT (nicotinamide N-methyltransferase), an enzyme regulating metabolism, fat storage, and NAD+ consumption.[4][27][6] NNMT methylates nicotinamide, a NAD+ breakdown product, diverting it from recycling and reducing available NAD+

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In animal-model work, NNMT inhibition with 5-Amino-1MQ has been associated with changes in adipose-tissue mass, in NAD+ pool sizes (because NNMT consumes nicotinamide that would otherwise feed the NAD+ salvage pathway), and in methyl-donor dynamics (because NNMT consumes S-adenosylmethionine)