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To assess whether R2-mediated TPRT could support the integration of a full-length gene cassette, we designed a donor RNA template encoding a functional gene circuit comprising a minimal synthetic promoter (pMinSyn), a GFP ORF (GFinP) and the nos terminator (NOSter), guided by previously established mammalian reporter systems 68,69

doi:10.1016/S1470-2045(09)70326-2

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