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The plate was then incubated at 37 C for 45 minutes in a shaker water bath then cooled and the absorbance of the pink colored chromophore was measured at 540 nm using ELISA reader, against a blank treated in a similar manner to the test but using 100l distilled water instead of the sample

48 Our study documents that GN diminishes MyD88, IRAK-1 and NF-Bp65 adaptor protein expressions