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The supernatant was also saved (tube 2) and the loosened epithelium was then manually scraped off into stock solution with 10% FBS (tube 3), and all cells were collected by centrifugation for 10 min at 225 g , 4 C (tubes 1, 2, and 3)

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and (iv) That the physician, in his or her reasonable medical judgment, must base those conclusions on one of the following determinations: (A) A medical condition has, or with a high degree of probability could have, precluded the employee from providing a sufficient amount of breath