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Western blot We used the NuPAGE electrophoresis and buffer system (Invitrogen) for immunoblot analysis of kidney samples or cellular lysates as described 48,49

Acetoacetyl-CoA is converted to hydroxymethyl glutaryl-CoA (HMG-CoA) by the mitochondrial rate-limiting ketogenic enzyme 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2, EC 2.3.3.10) (Figure 1)

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Illustration by Scott Holmes, CMI, Michael E