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His-tagged E2E1-foldon trimers were purified from 293 F supernatant using NiNTA agarose beads (Qiagen) and polished using a Superdex 200 Increase column in PBS

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Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were conducted to characterise the biological functions of differentially expressed genes (DEGs) in the TNF--treated NPCs

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LeClerque A, Wan H (2007) Novel dominant selection marker for the transformation of fungi

M., Villegas-Rivera, G., Rodriguez-Carrizalez, A