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Total RNA was reverse transcribed into cDNA using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific Cat # 4368814), followed by quantitative polymerase chain reaction (qPCR) with Power SYBR green PCR master mix (Thermo Fisher Scientific, Cat # 4367659) on the CFX96 Real-Time PCR System (BioRad)

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Toxicology 139 , 219232 (1999)

doi: 10.1016/j.yrtph.2008.01.004

The number of ookinetes per 1,000 erythrocytes was determined using an optical microscope and the drug inhibition percentage was calculated

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