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Cystine-free/GSH-supplemented = 6

Open questions What is the mechanism by which metabolic reprogramming affects the progression of the tumor

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Although these analyses are not specific to the central nervous system (CNS), they do offer clues to ongoing brain injury pathogenesis, as the increased permeability of the bloodbrain barrier (BBB) after TBI allows for greater exchange of CNS/peripheral solutes, and many of these metabolites are also detected in the brain parenchyma [34]

Examples of other 3D culture systems include RPTEC/TERT1 and Nki-2 cells that, when cultured in Matrigel or Matrigel mixed with collagen I, respectively, were able to further mature and form tubular structures as seen in vivo

Nat Struct Mol Biol 30(11):18061815 Hendricks JM et al (2023) Identification of structurally diverse FSP1 inhibitors that sensitize cancer cells to ferroptosis