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The genera such as Bacillus , Lactobacillus, Staphylococcus, Corynebacterium, Streptococcus, Prevotella, Rothia, Pantoea, Veillonella, Propionibacterium, Fusobacterium, Actinomyces, Lactobacillus, Sphingomonas, Marinilactibacillus, Oceanobacillus , and Porphyromonas were recognized in different STPs ( In our study, we focused on the moist STPs because high moisture content plays a significant role in the increased level of TSNAs by facilitating the growth of the microorganisms ( in vitro assay using epithelial cells (AMOL-III) from the oral leukoplakia of a Khaini user and treatment with aqueous extract of Khaini showed alteration in the expression of proteins involved in cell cycle regulation and DNA methylation, suggesting the role of Khaini in oral carcinogenesis ( Snus contains high levels of carcinogenic TSNAs (23.161.2 g/g) ( Qiwam products also retain a significant level of TSNAs (5.4322.2 mg/kg) (Tricker and Preussmann, 1989)

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Results LCs cause significantly greater adverse effects on airway epithelial function than cigarettes To assess the impact of LCs on human airway epithelia, we chronically exposed HBECs and (i) fixed cells to perform gross analysis by light microscopy and (ii) imaged live cells by XZ confocal microscopy