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Proteins in lysates of cells were separated by SDS-PAGE and immunoblotted using antibodies against the following proteins: SREBP2 (ab30682, 1:1000, Abcam, USA), HMGCR (ab242315, 1:1000, Abcam, USA), ABCA1 (NB400-105, 1:500, Novus Biologicals, USA), ABCG1 (ab52617, 1:1000, Abcam, USA), LXRa (ab176323, 1:1000, Abcam, USA), -actin (sc-47778, 1:1000, Santa Cruz Biotechnology, USA), LaminA/C (sc-7292, 1:1000, Santa Cruz Biotechnology, USA)

Takizawa et al

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