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Additional processing using ultrasound, pulsed electric field (PEF), microwave, high-pressure, supercritical CO 2 , or subcritical water at optimized conditions can enhance protein extraction yield and ameliorate its emulsifying and gelling properties by increasing protein unfolding and elevating its random structure

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We also treated old (26 months to 28 months) male mice with ELAM for 8 weeks by osmotic minipump, then isolated their hindlimb muscle mitochondria with ELAM (chronic treatment) (Fig

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This concentration works well for researchers who plan to stay at or below 1.7mg maintenance