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DNA vectors Expression vector for AHCY was obtained by amplifying the mouse AHCY ORF from the HA-AHCY vector 59 using the primers 5 -ATAGTCGACGCCACCATGTACCCATACGATGTT-3 and 5 -TATGCTAGCTTCAGTAGCGGTAGTGATCAGGCT-3 , then ligating the SalI/NheI-digested PCR product into the SalI/NheI-digested pSELECT-HYGRO-MCS vector (Invivogen) using Ligation high Ver.2 (Toyobo)

In animal studies, both routes have shown biological activity, but injection delivers the peptide systemically with greater predictability

As such, clinicians often face significant challenges of treating these symptoms in the outpatient setting

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This may be ascribed to the targeting ability of TPP