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Mol Cell 2017;66:517532.e519

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Moreover, introduction of the MESNA/diMESNA redox couple in the cleavage buffer allowed simultaneous on-column refolding of Ribonuclease A and intein-mediated cleavage to yield Ribonuclease A with a C-terminal MESNA-thioester

& Nishikawa, H

Functioning as an amphiphilic natural saponin, GA initiates self-assembly driven by its hydrophobic triterpenoid aglycone (18-GRA) and hydrophilic disaccharide glucuronic acid