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The constructs were validated by DNA sequencing

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resultant supernatant fractions were centrifuged at 1,000 g for another 15 min, followed by centrifugation of the supernatant at 3,000 g for 15 min

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Quan S, Yang L, Abraham NG, Kappas A (2001) Regulation of human heme oxygenase in endothelial cells by using sense and antisense retroviral constructs

Cells were washed with cold PBS and then fixed with 70 % cold ethanol for 15 min on ice